Generator

Part:BBa_K539731

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-24)

kivd (alpha-ketoisovalerate decarboxylase) under TetR repressible promoter

This is the coding sequence of kivd from Lactococcus lactis subsp. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination. Kivd play a vital rule in the isobutanol biosynthesis pathway. This part, kivd is under TetR repressible promoter that constitutively ON and repressed by TetR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None