Composite

Part:BBa_K539673

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0032)+ilvC+a RBS (B0032)+ilvD with RNA thermometer+tetR+terminator

Built in two weak expressing RBSs, ilvC, ilvD, 37’celcius regulator RBS, tetR and terminator (Part:BBa_K539641 with Part:BBa_K332033). It catalyze 2-Acetolactate into 2-Ketoisovalerate in isobutanol synthesis pathway and inhibit Ptet contained circuit when the temperature hit 37’C or higher. It could be applied in your interest part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4132
    Illegal AgeI site found at 611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197
    Illegal BsaI site found at 3029
    Illegal BsaI site found at 3286
    Illegal BsaI.rc site found at 1016


[edit]
Categories
Parameters
n/aa RBS (B0032)+ilvC+a RBS (B0032)+ilvD with RNA thermometer+tetR+terminator