Generator

Part:BBa_K539672

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated)+RBS+Alss with a RBS (B0032)+ilvC+a RBS (B0032)+ilvD

Built in Plac, three weak expressing RBSs, Alss, ilvC and ilvD (Part:BBa_K539631 with Part:BBa_K539641). It catalyze pyruvate into 2-Ketoisovalerate in isobutanol biosynthesis pathway and could be applied in your interest part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2148
    Illegal BsaI site found at 4980
    Illegal BsaI site found at 5237
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129
    Illegal BsaI.rc site found at 2967


[edit]
Categories
Parameters
n/apromoter (lacI regulated)+RBS+Alss with a RBS (B0032)+ilvC+a RBS (B0032)+ilvD