Generator

Part:BBa_K539671

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated)+RBS+Alss with a RBS (B0030)+ilvC+a RBS (B0030)+ilvD

Built in Plac, two strong and one weak expressing RBS, Alss, ilvC and ilvD (Part:BBa_K539631 with Part:BBa_K539642). It catalyze pyruvate into 2-Ketoisovalerate in isobutanol synthesis pathway and could be applied in your interest part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2564
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2150
    Illegal BsaI site found at 4984
    Illegal BsaI site found at 5241
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129
    Illegal BsaI.rc site found at 2969


[edit]
Categories
Parameters
n/apromoter (lacI regulated)+RBS+Alss with a RBS (B0030)+ilvC+a RBS (B0030)+ilvD