Generator

Part:BBa_K539652

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0032)+ilvC

Built in Plac, two weak expressing RBSs, Alss and ilvC (Part:BBa_K539631 with Part:BBa_K539622). It catalyze pyruvate into 2,3-Dihydroxy-isovalerate in isobutanol biosynthesis pathway and could be applied in your interest part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2148
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129
    Illegal BsaI.rc site found at 2967


[edit]
Categories
Parameters
n/apromoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0032)+ilvC