Intermediate

Part:BBa_K539621

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Taiwan   (2011-09-27)

a RBS (B0030)+ilvC

Built in weak expressing RBS (Part:BBa_B0030) and ilvC (Part:BBa_K539711). It catalyze 2-Acetolactate into 2,3-Dihydroxy-isovalerate in isobutanol biosynthesis pathway and could be applied in your interest part.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 1018


[edit]
Categories
Parameters
n/aa RBS (B0030)+ilvC