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Composite

Part:BBa_K539142

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Taiwan   (2011-09-19)

crtEBI+RNA thermometer+crtY+terminator

CrtEBI with RBS that is assembled by previous team is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). And then crtY(lycopene B-cyclase) converts lycopene to orange beta-carotene. The RNA thermometer that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Salmonella Enterica Tyhpy initiates translation at 37°C. Therefore crtY expresses significantly at 37°C. The native terminator is included.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1974
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1510
    Illegal NgoMIV site found at 1640
    Illegal NgoMIV site found at 4611
    Illegal AgeI site found at 725
  • 1000
    COMPATIBLE WITH RFC[1000]


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