Composite

Part:BBa_K539138

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Taiwan   (2011-09-18)

crtY (lycopene cyclase) with RNA thermometer and terminator

This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene. RNA thermometer is preceded with crtY. The RNA thermometer that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Salmonella Enterica Tyhpy initiates translation at 37°C. Native terminator is included in the part.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1218
  • 1000
    COMPATIBLE WITH RFC[1000]
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