Part:BBa_K538201

pLacI + RBS + Cpn60

LacI:Cpn60 K538201

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the Cpn60 protein from Oleispira antarctica (BBa_K538001) under control of the Lac operon's promoter, pLacI (BBa_R0010). When coexpressed with Cpn10 (BBa_K538000), it should strongly increase E. coli 's ability to grow at low temperature. Coexpression can be achieved by transforming an E. coli strain comprising this BioBrick with a Cpn10 generator, such as K538200 and K538300, or by transforming cells with an operon-like BioBrick containing both the Cpn10 and Cpn60 coding regions, such as K538210, K538211, K538310 and K538311.

Refer to the main pages of the coding region parts (Cpn10: BBa_K538000 and Cpn60: BBa_K538001) for more information on how they facilitate cold resistance in E. coli.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1096
  Illegal BamHI site found at 1162
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]