Plasmid

Part:BBa_K5371201

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-29)


pAbAi-GAL4-NH

GAL4_NH1574_V2 is a plasmid featuring upstream homologous region for mCherry integration, P2A for connection, GAL4 for signaling, and downstream homologous region for PRP11 integration. It integrates a signaling and reporting section into genome of Saccharomyces cerevisiae to report expression of GAL4, while regulating downstream genes through GAL/UAS system. It was applied into our project to report the expression level of the SIR2 located upstream, while regulating the expression of ERG20-MrBBS through GAL/UAS system.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 3600
    Illegal XbaI site found at 917
    Illegal PstI site found at 1560
    Illegal PstI site found at 4214
    Illegal PstI site found at 7688
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 3600
    Illegal PstI site found at 1560
    Illegal PstI site found at 4214
    Illegal PstI site found at 7688
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 3600
    Illegal BamHI site found at 48
    Illegal BamHI site found at 3331
    Illegal XhoI site found at 429
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 3600
    Illegal XbaI site found at 917
    Illegal PstI site found at 1560
    Illegal PstI site found at 4214
    Illegal PstI site found at 7688
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 3600
    Illegal XbaI site found at 917
    Illegal PstI site found at 1560
    Illegal PstI site found at 4214
    Illegal PstI site found at 7688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 348
    Illegal BsaI.rc site found at 2024
    Illegal BsaI.rc site found at 3213
    Illegal BsaI.rc site found at 5939
    Illegal BsaI.rc site found at 6998
    Illegal SapI site found at 713
    Illegal SapI site found at 4856
    Illegal SapI site found at 7150


[edit]
Categories
Parameters
None