Part:BBa_K5371102
SIR2-mCherry
The Sir2-linker-mCherry is a fusion protein in which mCherry, a red fluorescent protein, is attached to the C-terminus of Sir2, which is critical to the aging of Saccharomyces cerevisiae. It is designed to report the aging status and level of Saccharomyces cerevisiae while not affecting cell growth or aging. Fusion of mCherry to Sir2 ensures a 1 to 1 expression ratio, which helps with real-time quantification of Sir2 level, thus the aging status of yeast cells. The flexible linker prevents interference of protein functions, allowing normal fluorescence intensity and rDNA splicing silencing effect. The desired way of application is to integrate the part into the original location of the Sir2 in the genome using PRP11 as a downstream homologous region. Alternatives include knocking out the original Sir2 in the genome and removing the necessity of integration.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2038
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2038
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 823
Illegal BamHI site found at 261
Illegal BamHI site found at 809 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2038
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2038
- 1000COMPATIBLE WITH RFC[1000]
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