Part:BBa_K5371003

pRS42H

The plasmid backbone pRS42H is a yeast expression vector commonly used in synthetic biology and genetic engineering. It is designed to express genes in Saccharomyces cerevisiae and contains several key features that facilitate its use in various applications. The backbone includes a hygromycin resistance marker for selection in yeast, an ampicillin resistance marker for selection in bacteria, and a 2-micron origin of replication for high-copy maintenance in yeast cells. This plasmid is particularly useful for cloning and expressing genes in yeast, making it a valuable tool for metabolic engineering, protein production, and functional genomics studies.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 836
  Illegal XbaI site found at 7581
  Illegal PstI site found at 926
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 836
  Illegal PstI site found at 926
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 836
  Illegal BamHI site found at 3161
  Illegal XhoI site found at 3887
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 836
  Illegal XbaI site found at 7581
  Illegal PstI site found at 926
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 836
  Illegal XbaI site found at 7581
  Illegal PstI site found at 926
  Illegal NgoMIV site found at 2415
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2915
  Illegal BsaI.rc site found at 5751
  Illegal SapI site found at 4668