Composite

Part:BBa_K537014

Designed by: Ezio Fok   Group: iGEM11_WITS-CSIR_SA   (2011-09-16)

Promoter-Atrazine riboswitch-mCherry-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to the monomeric Red fluorescent reporter protein without a stop codon in between. The atrazine riboswitch-mRFP fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 52
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None