Coding

Part:BBa_K531003

Designed by: Qimeng Gao   Group: iGEM11_Grinnell   (2011-07-05)

Esp coding sequence from Staphylococcus epidermidis

Staphylococcus epidermidis, a commensal bacterium, was observed to secret the serine protease Esp to inhibit biofilm formation and nasal colonization by Staphylococcus aureus, a human pathogen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Untitled6.jpg

Caulobacter strain that has optimized esp gene codon showed better results in inhibiting S. aureus biofilm than the strain that has wild type gene codon (with the same BBa_K081005 promoter, p=0.002). It is plausible that high G/C content gene is expressed better than lower G/C content one in Caulobacter.

Untitled7.png

S. epidermidis that is producing Esp protein is indeed able to inhibit S. aureus biofilm, although S. epidermidis itself can form biofilm too.

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