Coding

Part:BBa_K530026

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-09-03)

GME

Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-D-Mannose to GDP-L-Galactose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Plasmid Map

GDP-Mannose-3,5-Epimerase Plasmid Map.png


Sequencing

GME Sequencing.png

This is the sequencing for GME. The areas of dubious identification are due to the gene being too long to be sequenced by only two primers.

[edit]
Categories
Parameters
None