Coding

Part:BBa_K530025

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-09-03)

Vtc2

Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-L-Galactose to L-Galactose-1-P.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 788
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Plasmid Map

GDP-L-Galactose Phosphorylase.png


Sequencing

GLGP Sequencing.png

This is the sequencing for GLGP. The areas of mismatch in the middle of the sequence are due to the fact that the gene is too long for only two primers to sequence adequately.

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Categories
Parameters
None