Regulatory

Part:BBa_K530003

Designed by: Daniel Wolozny   Group: iGEM11_Johns_Hopkins   (2011-08-04)

KRE9 Yeast Promoter

KRE9 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

Kre9p Plasmid Map.png


Sequencing

Kre9p sequencing.png


This is the sequencing for colony 4. Colony 4 is a perfect match with the genomic DNA promoter. Colony 6 was also tested, however the sequences did not match.

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