Measurement

Part:BBa_K528021

Designed by: Pawel Urbanski   Group: iGEM11_Warsaw   (2011-09-19)

Expression Adapter fuse with E0030 YFP protein

Construct design

This device was used to measure expression level obtained from BBa_K528018 fused with Yellow Fluorescent Protein BBa_E0030. We have measured fluorescence level and compared it to BBa_K528010 B0034+YFP reference construct.

We have obtained the following results:

Part NameDescriptionExpression level as %B0034+YFPStd Dev.
BBa_K528021BBa_K528018+YFP measurement device20,37%3,21%
referenceBba_J23102+B0034+YFP (100% reference construct)100,00%2,61%


According to design BBa_K528018 has strength 22,86% relative to BBa_B0034 with given protein. This means that our expression adaptor works according to the design.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None