Part:BBa_K527001

Biosynthesis/Oximes/CYP79A2/Plug 'n' Play

This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.

CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of a cyanogenic glycoside. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.

BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid (BBa_K678023) due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 165
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 432
  

References

• Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.

• Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.