Part:BBa_K5268053

Lac-S12-4

Lac-S12-4

Lac-S12-4

Protein sequence:

MPSANRARASALPRRSFLTAAGLLAAGAALEAYGPEQLRVFESSAPAGRETSQPSTVAADGVVQLFAAPDVVDLGGRSAKTWTFNGSLPGPELRVPAGQLLRVDFENRLPAPTSIHWHGIAIRNDMDGVPGFTQRAIAPGSRFLYEFTVPDPGTYFFHPHAGVQLDRGLYGVLIVEDPAEPGRYDREAVVVLDDWTDGVGETPDRILARLLANGMDHSKMPGMGHSMGGMHHGAGMAMATATRPLGDDTGDIRYPLYLANGRQARSPATLSARPGERVRLRVVNAAADTAFRLALGGHRLTVTHADGFPVRPVTGDALLLGMGERYDALVQLHDGAFPLVAVAEGKSAQALAVVQTGRGAAPGAGVRPNELRRRLLTADDLEAAADVDLGPATPHRIYRLVLDGNMATYRWTINGRTMHGGAPLKVRDGERVRLVFDNRSTMFHPMHLHGHTFQLRRTGPRDPGPRKDTVIVRPGERVMVDFDAANPGRWMLHCHNAYHQAGGMMTELVYDR

Plasmid Profile:

PCR gel charts:

IMAGE

M: Marker, 2000 bp; Line13: Lac-S12-4（1539 bp）

Results of Western Blot:

M：Maker; C: CK; Line7: Lac-S12-4,55.28kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.238%

Line1: CK; Line7: Lac-S12-4;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image: Fourier Diagram:

Plastic Film(Carbonyl index(CI):0.18%)

(None)

Plastic Microspheres(Carbonyl index(CI):None)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 853
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 614
  Illegal XhoI site found at 20
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 76
  Illegal NgoMIV site found at 135
  Illegal NgoMIV site found at 139
  Illegal NgoMIV site found at 327
  Illegal NgoMIV site found at 798
  Illegal NgoMIV site found at 1090
  Illegal NgoMIV site found at 1501
  Illegal AgeI site found at 1313
  
• 1000
  COMPATIBLE WITH RFC[1000]