Part:BBa_K5268052

Lac-S12-3

Lac-S12-3

Lac-S12-3

Protein sequence:

MKVFDLETAVTNWYILPEVRTNAYAFNGQVPGPRLRITEGDSVRINVTNRLPESTTVHWHGLILPNAMDGPAEITQEPIQPGATYTYEFVAQQRGTFFYHSHDRVDRQQALGLYGALLIDPREPPTQPVYDKELVVQLQEWLYRDDYTFPSMPMEGAMPNFFTINGKAYPDTETVNLRVGERLLVRFIGSNSGFIHPMHIHGGPFRIVETDGYPLSEAAQLVKDTVNVGPGERYDVIWEAREPGKWLLHCHINHHVTNDSREEQGGGGLMLILNVSGP

Plasmid Profile:

PCR Gel Charts:

M: Marker, 2000 bp; Line23: Lac-S12-3(837 bp)

Results of Western Blot:

M：Maker; C: CK; Line10: Lac-S12-3,31.43kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.630%

Line1: CK; Line5: Lac-S12-3;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image: Fourier Diagram:

Plastic Film(Carbonyl index(CI):2.27%)

Plastic Microspheres(Carbonyl index(CI):2.90%)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]