Lac-S12-2

Protein Sequence:

MPEEHGETRSDITHTATQSSDGRAGHEDHAAMNHGGMHHEMKPNVTRPQLAAVGLLTVLALIASVVIPAMFVNLTYSAEEVGGAVMPPGMVMSRDTPAAAMREMAAIDPGEVSYEAPAGARGDRSLEPKIEDGVKVFDLGVSVVEWNILPDQSVEAYAFNRQVPGPRIRVTEGDRVRINVANDLPEATSVHWHGMILPNAMDGAADVTQKPIEPGETFPYEFTAKEAGTYFYHSHKEPDRQQGLGMYGALIVDPKDPARDAAYDYDQEMVVQLQEWLERDGYTYPAMTMEGALPNYFTINGKSYPETETVNMEVGERLRIRFIGSNNNFVHPMHVHGGPFEIVETDGNVVPDDARLLKDTVNVGPGERYDVVWEAREPGKWLVHCHIPHHTTNDNTEQEGGGGLMAVINVT

Plasmid Profile:

PCR gel charts:

M: Marker, 2000 bp; Line8: Lac-S12-2（1236 bp）

Results of Western Blot:

M：Maker; C: CK; Line9: Lac-S12-2,45.18kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.929%

Line1: CK; Line2: Lac-S12-2;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image:

Fourier Diagram:

Plastic Film(Carbonyl index(CI):2.73%)

Plastic Microspheres(Carbonyl index(CI):2.90%)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 817
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 817
Illegal NotI site found at 292
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 956
Illegal XhoI site found at 829
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 817
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 817
Illegal AgeI site found at 368
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 333
Illegal BsaI.rc site found at 646
Illegal BsaI.rc site found at 916
Illegal BsaI.rc site found at 1030
Illegal SapI.rc site found at 7

Part:BBa_K5268051

Lac-S12-2