Part:BBa_K5268050

Lac-S12-1

Lac-S12-1

Lac-S12-1

Protein Sequence:

MGLGLAGGVTLALPFSASACSGSGEGSTGTLLPSKAKLPEPFRIPLPIPPVLEPVRTDAGADYYEMTQKAGRMGILPGFQTEVWGYDGIFPGPTIESRSGRKIVVRQRNELLVPVSTHLHGGRTPPGSDGYPTDLIPPKKMVHDHSMDGHGSMDLGGSRHSKDYVYPLEQQAATLWYHDHRMDFTGPQVWRGLAGFHIIRDDEEDTLPLPKGDKDVPLMICDRSFDEDGSFLYPSLDPSLQGKPGVEDDYMDGVLGDTILVNGAPWPVLEVSATKYRFRILNASNARRYELTLEPGNDAPFVQVGSDGGLLGAPILHRAIRIAQAERFDVVIDFSRYDVGTEVTLNNRMGEGKTASVMRFRVIRKEKDDSAVPRRLSDMKEFQELEESRARRTREFRFTRVGDNGRVMWGINGEPFDPDRMAAKPELGTTEVWHLRAAPAAHPIHLHLVHFKVLSRNGGKPLSTDAGWKDTVDLTSGEEARVLVRFDGYRGRYVFHCHNLEHEDMMMMANFETV

Plasmid Profile:

PCR Gel Charts:

M: Marker, 2000 bp; Line7: Lac-S12-1(1545 bp)

Results of Western Blot:

M：Maker; C: CK; Line3: Lac-S12-1,57.2kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0.876%

Line1: CK; Line2: Lac-S12-1;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image: Fourier Diagram:

Plastic Film(Carbonyl index(CI):3.16%)

Plastic Microspheres(Carbonyl index(CI):3.65%)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1
  Illegal PstI site found at 63
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal PstI site found at 63
  Illegal NotI site found at 302
  Illegal NotI site found at 368
  Illegal NotI site found at 1475
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal BamHI site found at 714
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1
  Illegal PstI site found at 63
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1
  Illegal PstI site found at 63
  Illegal NgoMIV site found at 1320
  Illegal AgeI site found at 165
  
• 1000
  COMPATIBLE WITH RFC[1000]