Part:BBa_K5268046

Lac-PM3-1

Lac-PM3-1

Lac-PM3-1

Protein sequence:

MINTTLCYIENDNKYLMLHRNKKQNDLNAAKWIGVGGKFEPGETVEECLVREVFEETGLTLTKFVYVGMIKFVSDIYEDEDMYLFKGTEFTGEVKEDCREGTLKWVNSEEVLDLPTWEGDRYFLKPLLDGCTGLNMTVCYEGDKLVKVVDSTQQVEIDSSSLISSPHGFSTRIGGVSDGVYGSLNLGMNRGDEQARVTENWRRFMEKCGIGYRPFVCGKQVHGNNVHVVTFEDARWAYGPGELIEADGYVTKEKELPIAIFTADCVPVLLEDKEAGVIGVIHCGWRSTVADIEGKTIEKMLELGASTSNINVAIGPAIEQCCFEVGPEVVEAANALLRKGTSISVNSKGFKTKLDVGAEESEFAVPGAPGKYMLDLKAVVERRMIQLGISSNNIEKVGMCTMCHPDRYYSHRYYSQGDSNQSRGSLATVIMMK

Plasmid Profile:

PCR gel charts:

M: Marker, 2000 bp; Line9: Lac-PM3-1（1302 bp）

Results of Western Blot:

M：Maker; C: CK; Line11: Lac-PM3-1, 48kDa

Loss of weight (rate of degradation of original membrane weight, etc.): 1.062%

Line1: CK; Line2: Lac-PM3-1;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image:

Fourier Diagram:

Plastic Film(Carbonyl index(CI):3.46%)

Plastic Microspheres(Carbonyl index(CI):5.35%)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]