Part:BBa_K5268042

Lac-NJ2-1

Lac-NJ2-1

Lac-NJ2-1

protein sequence:

MSARVSRRGFLGLLGGAAALAALPACGPGATAGSTGEVLASAVPLPQPYRVPLPVPPVARPTGVDDTGAQLYRVTQRVAEAEILPGVRTPILGYDGIFPGPTFETRRGEAVVVRHRNELPVPTVVHLHGGHTPAEHDGWPLDLVLPAGDSRDWQGHHSMVGDVAAGEREHRYPNDQRAATLWYHDHRMDFTGPAVYRGLAGFHIVRDDVEDALPLPRGERELPLMICDRAFAADGSFAYPGLDQGMRSLPGVEHDWMEGVLGDVVLVNGAPWPVHEVDAARYRLRLLNASNARRYRLVLTVPGGPDVPFVQIGSDGGLLPAPVEHQAIELASGERFDVLVDFSVLPVGTEVTMVNGLDGGTAGRVMRFRVVRTARDDSRVPSRLAEFEPLVPPDGAPQRTFRFSRGPVGDHRGWTINDTSFDPDTSLADIRLGETEVWRFVTDVHHPVHVHLDAFQVLRRGSGGPLDLERGWKDTVDVRPAEIVDVAVRFSDHAGRFVFHCHNLEHEDMAMMATIRTV

plasmid mapping:

PCR gel charts:

注：M：Marker，2000 bp；Line12：Lac-NJ2-1（1557 bp）

Results of Western Blot:

M: Marker; C: CK; Line11: Lac-NJ2-1, 56.32kDa

Loss of weight (rate of degradation of original membrane weight, etc.)：0.814%

Line1: CK; Line3: Lac-NJ2-1;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

scanning electron microscope image:

Fourier diagram:

Plastic Film(Carbonyl index(CI):3.34%)

Plastic Microspheres(Carbonyl index(CI):4.01%)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 441
  Illegal NgoMIV site found at 963
  
• 1000
  COMPATIBLE WITH RFC[1000]