Part:BBa_K5268041

Lac-NJ-1

Lac-NJ-1

Lac-NJ-1

Protein Sequence:

MAGFHLAFNAFDTGNESTGFRLPSFPEFDIPLILNDKLFHPTTGLLAFDTFRFDGLLGDRFMVNGKIQPFFEAKKRRYRFRVLNGGPSRVHRFVLTNPHNLTQQIPVLVISSDGNLLGRPIEVTSYPVSVAERVDIIVDFKKIADRFGASRVRLENRLEQTNGQGPTGNIRAAGQGDQLMEFRLTGGAVTDNSFDPEPVSFPRVPAAANDAVFAPITLPDLNQMKNEVRITRTFDFERDRDGQWVINGELMDCTRSRFTVQKNSTERWILKNRQDDWSHPIHIHLEEFRIVSRDGGDFSFRTNTDSDYGSRESGSRSSDATIRPGDIEFGRKDVLRLGEEEVEIIMRFRDFTGVYPMHCHNTIHEDHQMMLLWRVADQGDFKTEP

Plasmid Profile:

PCR Gel Charts:

M：Marker，2000 bp；Line14：Lac-NJ-1, 1158 bp

Results of Western Blot:

M: Marker; C: CK; Line5: Lac-NJ-1, 43.99kDa

Loss of Weight (rate of degradation of original membrane weight, etc.): 0%

Line1: CK; Line4: Lac-NJ-1;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

Scanning Electron Microscope Image:

Fourier Diagram:

Plastic Film(Carbonyl index(CI):0.26%)

(None)

Plastic Microspheres(Carbonyl index(CI):(None))

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1
  Illegal NgoMIV site found at 618
  Illegal AgeI site found at 597
  
• 1000
  COMPATIBLE WITH RFC[1000]