Part:BBa_K5268037

AlkB-S102-2

AlkB-S102-2

AlkB-S102-2

protein sequence:

MPSLGFGAWGLVEATGLSVFWYLGPIVVFIVVPAVDLIAGLDRSNPPDDVIEALENDRYYRWITYLFLPIQYAALIWACWMFTEPSWAPAMSVVDKVGLALTIGTIAGIGINTAHELGHKKESHERWLAKIALAQSFYGHFYIEHNRGHHVRVATPEDPASSRVGESFYAFWPRTVSGSLKSAWNLEKPRFKRKGKSHWSIKNDVLNAWLMSVVLWGALIAVFGIGILPFLIIQAIVGFSLLEVVNYLEHYGMLRQRTGPDGTGRYERVQPSHSWNSNNIATNVLLYHLQRHSDHHANPTRRYQTLRDFEESPVLPTGYAGLIILALIPPLWFKVMDKRVLAHFDGDITRANIQPSKREKILKKYGAPATTAPEVAA

plasmid mapping:

PCR gel charts:

注：M：Marker，2000 bp；Line3：ALKB-S102-2（1134 bp）

Results of Western Blot:

M: Marker; C: CK; Line12: AlkB-S102-2, 42.44kDa

Loss of weight (rate of degradation of original membrane weight, etc.)：0.0867%

Line1: CK; Line4: AlkB-S102-2;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

scanning electron microscope image:

Fourier diagram:

Plastic Film(Carbonyl index(CI):1.08%)

(None)

Plastic Microspheres(Carbonyl index(CI):(None))

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 871
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 871
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 871
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 871
  
• 1000
  COMPATIBLE WITH RFC[1000]