Part:BBa_K5268035

AlkB-NJ-3

AlkB-NJ-3

AlkB-NJ-3

protein sequence:

MTTDTPATETPATTAEPATGAPARWHDPKRHLWLGGLLAWLTGWNAMWWMGPLWIFVVVPLLDTLAGTDASNPPDWAVPQLESDRYYRWCVYLYIPLQFVGFLWGAAVVAGGTLSPLSQLGLALTVGTVAGVGINTAHELGHKRERVERRLSKVALAQSGYGHFYVEHNRGHHSNVSTPEDPASARFGESFWAFLPRTVVGSLRSAWALAAGAQRRRGRRVWSPGNDVLNAWALSVVLFGATIALFGPVVIPYVVVQAVFGFSLLEVVNYIEHYGLLRQRVANGRYERCRPEHSWNSNNIVSNLVLYHLERHSDHHANPTRRYQSLRHFDEAPQLPSGYALMIGLAYLPPLWRRVMDHRVLAHYGGDITRANIHPPARPRVLARYAPDA

plasmid mapping:

PCR gel charts:

注：M：Marker，2000 bp；

Results of Western Blot:

M: Marker; C: CK; Line10: AlkB-NJ-3, 43.47kDa

Loss of weight (rate of degradation of original membrane weight, etc.)：0.1127%

Line1: CK; Line5: AlkB-NJ-3;

Polyethylene film weight loss (0.01 < P ＜ 0.05 labelled *, 0.001 < P ＜0.01 labelled **, P ＜ 0.001 labelled ***, P > 0.05 indicates no statistical significance labelled ns)

scanning electron microscope image:

(None)

Fourier diagram:

Plastic Film(Carbonyl index(CI):1.50%)

(None)

Plastic Microspheres(Carbonyl index(CI):(None))

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 47
  Illegal PstI site found at 295
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 47
  Illegal PstI site found at 295
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 47
  Illegal PstI site found at 295
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 47
  Illegal PstI site found at 295
  
• 1000
  COMPATIBLE WITH RFC[1000]