Part:BBa_K525008

Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB

Fusion protein of SbpA || BisdA || BisdB (RFC 25) for S-layer immobilized, cell-free BPA degradation after expression in E. coli using T7 promoter when combined with a ferredoxin-NADP⁺ oxidoreductase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 104
  Illegal BglII site found at 221
  Illegal BamHI site found at 3792
  Illegal BamHI site found at 4530
  Illegal XhoI site found at 1996
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 76
  Illegal AgeI site found at 4783
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 493
  Illegal BsaI.rc site found at 622