Coding

Part:BBa_K525007

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-12)

Fusion protein of S-Layer SgsE with T7/lac and BisdA and BisdB

Fusion protein of SgsE || BisdA || BisdB (RFC 25) for S-layer immobilized, cell-free BPA degradation after expression in E. coli using T7 promoter when combined with a ferredoxin-NADP+ oxidoreductase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
    Illegal BglII site found at 1022
    Illegal BamHI site found at 3000
    Illegal BamHI site found at 3738
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 3991
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1657


[edit]
Categories
//chassis/prokaryote/ecoli
//function/degradation/bisphenol
//proteindomain/degradation
//proteindomain/internal
//rnap/bacteriophage/t7
Parameters
biologyS-Layer
chassisE. coli
controlPT7
originGeobacillus stearothermophilus, Sphingomonas bisphenolicum AO1