Project

Part:BBa_K525002

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-11)

Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac

Fusion protein of SbpA || Red || BisdA || BisdB (RFC 25) for S-layer immobilized, cell-free BPA degradation after expression in E. coli using T7 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal BamHI site found at 4539
    Illegal BamHI site found at 5277
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 5530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622
    Illegal BsaI.rc site found at 3874


[edit]
Categories
//chassis/prokaryote/ecoli
//function/degradation/bisphenol
//proteindomain/degradation
//proteindomain/internal
//rnap/bacteriophage/t7
Parameters
biologyS-Layer
chassisE. coli
originLysinibacillus sphaericus, Escherichia coli, Sphingomonas bisphenolicum AO1