DNA

Part:BBa_K524203

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2012-08-26)

pSC101 heat sensitive origin of replication with deletion in par locus

A derivative of pSC101 origin of replication, this heat sensitive origin has the par locus deleted, therefore allowing a rapid loss of plasmid even under permissive temperatures.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/component/origin
Parameters
None