Generator

Part:BBa_K524202

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2012-08-19)


IPTG inducible Cre recombinase

Generator of Cre recombinase. A leaky inducible promoter R0010 and strong RBS B0034 were employed to drive strong expression of Cre recombinase upon addition of IPTG to medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None