Reporter

Part:BBa_K524006

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2011-10-04)

RBS + split sfGFP1-10

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. The amino acid residues 214 and 215 of sfGFP have been reported to undergo spontaneous complementation to restore green fluorescence.

Usage and Biology

When driven by an appropriate promoter, the product of this biobrick should complement with the product of biobrick sfGFP11 BBa_K524007 to give green fluorescence under UV.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 31


[edit]
Categories
Parameters
None