Generator

Part:BBa_K524004

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2011-10-03)

pir gene

The pir gene encodes the π protein. Initiation of replication at ori-γ or other R6K origins of replication requires the π protein in trans. Increasing its expression to double the natural concentration produced would, however, effectively shut down the replication. Expression of π protein is autogenously regulated.

Usage and Biology

The presence of pir gene allow maintenance of plasmids with R6K origins as their sole origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None