Composite

Part:BBa_K5217016

Designed by: qing kong   Group: iGEM24_BGI-MammothEdu-East   (2024-09-26)


pGPM1_tLsCl_PGK1t

pGPM1 is a promoter. It is added before the gene tLsCl and followed by the PGK1 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 2011
    Illegal PstI site found at 943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 2011
    Illegal NheI site found at 1416
    Illegal PstI site found at 943
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 2011
    Illegal BglII site found at 181
    Illegal BglII site found at 1472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 2011
    Illegal PstI site found at 943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1063
    Illegal EcoRI site found at 2011
    Illegal PstI site found at 943
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1459


[edit]
Categories
Parameters
None