Coding

Part:BBa_K5217007

Designed by: CHUQIAO LIN   Group: iGEM24_BGI-MammothEdu-East   (2024-09-25)


tLSCL-Limonene coding sequence

tLSCL is the gene responsible for encoding the enzyme limonene synthase, which catalyzes the conversion of geranyl diphosphate to limonene. Limonene is a monoterpene commonly found in citrus fruits and is used in flavoring, fragrances, and solvents. The gene originates from Citrus limon


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 295
    Illegal EcoRI site found at 1243
    Illegal PstI site found at 175
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 295
    Illegal EcoRI site found at 1243
    Illegal NheI site found at 648
    Illegal PstI site found at 175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 295
    Illegal EcoRI site found at 1243
    Illegal BglII site found at 704
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 295
    Illegal EcoRI site found at 1243
    Illegal PstI site found at 175
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 295
    Illegal EcoRI site found at 1243
    Illegal PstI site found at 175
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 691


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Categories
Parameters
None