Part:BBa_K5217002
TDH3 promoter
The TDH3 promoter is a well-characterized, constitutive promoter from Saccharomyces cerevisiae that regulates the expression of the TDH3 gene, one of the three genes (TDH1, TDH2, TDH3) encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH). GAPDH is a critical enzyme in the glycolysis pathway, converting glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate. The TDH3 promoter is widely used in metabolic engineering and synthetic biology due to its robust and continuous activity across different environmental and metabolic conditions, particularly in glucose-rich media.
The TDH3 promoter is a preferred choice for high-level expression of foreign genes, as it provides stable and efficient gene expression without the need for specific induction. This promoter's strength and utility make it suitable for industrial applications, where high-level production of proteins, enzymes, or other metabolites is required. Studies have shown that the promoter sequence contains multiple upstream activation sites that contribute to its strong activity, and it is often employed in yeast metabolic pathways for efficient bioproduction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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