Part:BBa_K519022

Promoter(High) and RBS with SmtA

This part consists of a high constitutive promoter expressing SmtA protein without PduP1-18.

Previously , both SmtA and PduP1-18-fMT were expressed under a relatively low constitutive promoter (BBa_J23117). This time, we changed this promoter to a high constitutive promoter (BBa_J23100) and observed the growth in 300 µM Cd(II) medium, where growth of E. coli could not be observed before. However, E. coli with a high constitutive promoter could tolerate the Cd(II) containing medium better than the E. coli with low constitutive promoter (Fig.12, Fig.13). This suggests that the tolerance could be due to the amount of metallothionein being expressed, with a high constitutive promoter, E. coli could produce more metallothioneins and therefore could resist Cd(II) better.

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Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 154
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 154
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 154
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 154
  Illegal AgeI site found at 182
  
• 1000
  COMPATIBLE WITH RFC[1000]