Composite

Part:BBa_K519018

Designed by: Kotone Miyake   Group: iGEM11_Tokyo-NoKoGen   (2011-10-13)


Promoter and RBS with SmtA

P(J23109)-RBS(B0034)-SmtA(K519010)-Term(B0015)

We have expressed SmtA under a constitutive promoter. By comparing the growth rate by looking at OD595, of WT E.coli and SmtA expressing E. coli, we have found that E. coli expressing SmtA could grow better in a medium of high Cd(II) concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 154
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 154
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 154
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 154
    Illegal AgeI site found at 182
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None