Composite
Part:BBa_K519018
Designed by: Kotone Miyake Group: iGEM11_Tokyo-NoKoGen (2011-10-13)
Promoter and RBS with SmtA
P(J23109)-RBS(B0034)-SmtA(K519010)-Term(B0015)
We have expressed SmtA under a constitutive promoter. By comparing the growth rate by looking at OD595, of WT E.coli and SmtA expressing E. coli, we have found that E. coli expressing SmtA could grow better in a medium of high Cd(II) concentration.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 154
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 154 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 154
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 154
Illegal AgeI site found at 182 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |