Composite

Part:BBa_K519016

Designed by: Kotone Miyake   Group: iGEM11_Tokyo-NoKoGen   (2011-09-30)

P(J23109)-GlpF


P(J23109)-Glpf(K190028)

Constitutive promoter added to GlpF, an aquaglycerol porin that can trasport arsenic ion through the cell membrane, that was submitted by Team Groningen in iGEM2009. It has a symmetric structure with 4 independent GlpF channels. It transports substances depending rather on the molecular size than the chemical structure. It transports variety of non charged substances such as alcohol, glycerol, arsenic and antimone. It is known to be unable to transport carbon sugars and ions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 324
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None