Part:BBa_K519015

Promoter and RBS with PduP1~18 fused SmtA

P(J23117)-RBS(B0034)-PduP1~18+SmtA+Term(B0015)

A cadmium binding protein metallothionein SmtA (cloned from Synechococcus sp. PCC7942) was fused to PduP1~1. PduP protein (propionaldehyde dehydrogenase) is an enzyme utilized inside 1,2-propanediol utilization bacterial micro compartment. N-terminal ends of PduP is known to be needed to be taken into Pdu BMC. The short N-terminal peptide is necessary for packing enzymes into the pdu BMC. It has been reported that the deletion of 10 or 14 amino acids from the N terminus of the enzyme resulted in impaired packaging. On the other hand, fusion of the 18 N-terminal amino acids from PduP to GFP, GST, or maltose-binding protein resuted them to be encapsulated in BMC. The PduP1~18 protein can be fused to an aiming compound which can then be brought into the BMC.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 214
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 214
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 214
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 214
  Illegal AgeI site found at 242
  
• 1000
  COMPATIBLE WITH RFC[1000]