Composite

Part:BBa_K519013

Designed by: Kotone Miyake   Group: iGEM11_Tokyo-NoKoGen   (2011-09-30)

Promoter and RBS with PduP1~18 and Term.


P(J23117)-RBS(B0034)-PduP1~18-Term(B0015)

Terminator was added at the end of PduP1~18, to our registered part BBa_K519012 This part constitutes of a PduP (propionaldehyde dehydrogenase) protein with the N-terminal ends known to be needed to be taken into Pdu BMC. The short N-terminal peptide is necessary for packing enzymes into the pdu BMC. It has been reported that the deletion of 10 or 14 amino acids from the N terminus of the enzyme resulted in impaired packaging. On the other hand, fusion of the 18 N-terminal amino acids from PduP to GFP, GST, or maltose-binding protein resuted them to be encapsulated in BMC. The PduP1~18 protein can be fused to an aiming compound which can then be brought into the BMC.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None