Coding

Part:BBa_K5182024

Designed by: Zhao Ze zheng   Group: iGEM24_SJTU-BioX-Shanghai   (2024-09-29)


Light-controlled gene therapy composite element based on XPC disease 1

In this composite part, the UAS sequence can be recognized by the Gal4 sequence, the XPC is used to express the normal XPC protein in the patient, and the P2A sequence is used to promote the translation of the same mRNA strand for the expression of GFP and RUP2, and the expression of RUP2 will have a negative feedback effect on the system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2682
    Illegal EcoRI site found at 3266
    Illegal EcoRI site found at 4105
    Illegal PstI site found at 431
    Illegal PstI site found at 1080
    Illegal PstI site found at 1170
    Illegal PstI site found at 1200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2682
    Illegal EcoRI site found at 3266
    Illegal EcoRI site found at 4105
    Illegal PstI site found at 431
    Illegal PstI site found at 1080
    Illegal PstI site found at 1170
    Illegal PstI site found at 1200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2682
    Illegal EcoRI site found at 3266
    Illegal EcoRI site found at 4105
    Illegal BglII site found at 926
    Illegal BamHI site found at 2960
    Illegal BamHI site found at 3649
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2682
    Illegal EcoRI site found at 3266
    Illegal EcoRI site found at 4105
    Illegal PstI site found at 431
    Illegal PstI site found at 1080
    Illegal PstI site found at 1170
    Illegal PstI site found at 1200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2682
    Illegal EcoRI site found at 3266
    Illegal EcoRI site found at 4105
    Illegal PstI site found at 431
    Illegal PstI site found at 1080
    Illegal PstI site found at 1170
    Illegal PstI site found at 1200
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2049
    Illegal BsaI.rc site found at 2057
    Illegal BsaI.rc site found at 3448
    Illegal SapI site found at 1744
    Illegal SapI.rc site found at 386
    Illegal SapI.rc site found at 657


[edit]
Categories
//awards/composite_part
Parameters
biology