Part:BBa_K5182009
Self-cleaving peptides, derived from foot-and-mouth disease virus, have an average length of 18-22
The P2A peptide is a short peptide sequence (typically about 18-22 amino acids) that facilitates a "cleavage" effect between two proteins translated from the same mRNA, even though they are encoded consecutively on the mRNA. Although this "cleavage" occurs during translation, the actual separation is not through hydrolysis but rather due to the skipping of peptide bonds in the translation mechanism, resulting in the nascent proteins being split into two independent parts. When the P2A sequence is inserted between two target genes, the translation mechanism allows these genes to be translated into two distinct proteins without the need for additional protease processing. The C-terminus of the first protein will contain a residual fragment of P2A (an extra proline residue), while the second protein is completely independent. In this system, P2A is used to co-express XPC and RUP2 within the same sequence to produce the proteins.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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