Protein_Domain

Part:BBa_K5181012

Designed by: Eeshani Umesh Abhyankar   Group: iGEM24_IISER-TVM   (2024-09-01)


BM_linker

This part codes for a linker sequence used to make CPR-P450 fusion protein. The sequence is obtained from a soluble P450 enzyme from Bacillus megaterium (P450BM-3), which was previously shown to contain both heme and reductase domains on a single polypeptide (self-sufficient). Because of this feature, P450BM-3 exhibits the highest turnover rate among known P450 enzymes. We used this unique linker sequence to design our Sandalwood P450-CPR fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None