Generator

Part:BBa_K518009

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-26)


AspA expression cassette (no promoter)

AspA is an aspartate ammonia-lyase which catalyzes the equilibrium of aspartate and fumarate + ammonium ion. With enough amount of substrate, it produces aspartate. Aspartate can be used as a chemoattractant for Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 91
    Illegal BglII site found at 1556
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 983
    Illegal AgeI site found at 512
    Illegal AgeI site found at 1364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1023


[edit]
Categories
//cds/biosynthesis
Parameters
None