Generator

Part:BBa_K518004

Designed by: Masato Ohgishi   Group: iGEM11_UT-Tokyo   (2011-09-15)

IPTG-inducible L-Asp producing device

AspA is an aspartate ammonia-lyase which catalyzes the equilibrium of aspartate and fumarate + ammonium ion. With enough amount of substrate, it produces aspartate. Aspartate can be used as a chemoattractant for Escherichia coli. In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 154
    Illegal BglII site found at 1619
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1046
    Illegal AgeI site found at 575
    Illegal AgeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1086


[edit]
Categories
//function/motility
Parameters
None