Generator
Part:BBa_K518004
Designed by: Masato Ohgishi Group: iGEM11_UT-Tokyo (2011-09-15)
IPTG-inducible L-Asp producing device
AspA is an aspartate ammonia-lyase which catalyzes the equilibrium of aspartate and fumarate + ammonium ion. With enough amount of substrate, it produces aspartate. Aspartate can be used as a chemoattractant for Escherichia coli. In this device, AspA CDS, flanked with RBS and terminator, is under control of lacI-regulated strong promoter. IPTG can be used to induce aspartate production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 154
Illegal BglII site found at 1619 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1046
Illegal AgeI site found at 575
Illegal AgeI site found at 1427 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1086
[edit]
Categories
Parameters
//function/motility
None |