Coding
Part:BBa_K518003
Designed by: Masato Ohgishi Group: iGEM11_UT-Tokyo (2011-09-15)
LexA - a repressor for LexA-regulated SOS promoters -
Escherichia coli and other organisms have a gene cluster activated in case of emergency, which is called "SOS genes". It is well-documented that in E. coli, most of those SOS genes are repressed by LexA protein. When the cell is exposed to excess stress, RecA is up-produced, which promotes the self-degradation of LexA. As a result, many LexA-regulated genes become expressed. Combined with RecA and SOS promoters (for instance, recAp and sulAp), a stress-responsive gene-expression circuit was assembled.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 684
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
None |