Measurement

Part:BBa_K516330

Designed by: Susanna Zucca, Nicolo' Politi, Davide Bianchini, Paolo Magni   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

mRFP producer controlled by 3OC6-HSL with RBS B0030


The luxR based receiver (BBa_K081022) controls the production of mRFP (BBa_K516030).
In presence of cell-cell signaling molecule 3OC6-HSL, a LuxR transcription factor (BBa_C0062) is able to activate a Plux promoter (BBa_R0062).
LuxR production is driven by a cI lambda regulator (BBa_R0051). If used in a cell containing cI repressor (BBa_C0051) Plambda promoter is turned off, and the production of LuxR transcription factor is inhibited. In absence of the cI repressor Plambda promoter behaves as a strong constitutive promoter.
This is a biosensor which receives 3OC6-HSL concentration as input and returns mRFP intensity as output.

This measurement device belongs to a set of four biosensors that differ in RBS efficiency of the reporter system useful to modulate the output range.
(BBa_K516331 ; BBa_K516332 ; BBa_K516334)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1553
    Illegal AgeI site found at 1665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 912


[edit]
Categories
Parameters
n/amRFP producer controlled by 3OC6-HSL with RBS B0030